期刊
JOURNAL OF IMMUNOLOGY
卷 174, 期 7, 页码 4010-4018出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.174.7.4010
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The commitment of naive T cells to proliferate is a function of the strength and duration of stimuli mediated by the TCR and coreceptors. Ranges of 2-20 h of stimulation have been reported as necessary in vitro. Whether T cells actually experience uninterrupted stimulation for such long periods under physiological conditions is controversial. Here we ask whether commitment to proliferate requires continuous stimulation, or can T cells integrate intermittent periods of stimulation. T cells were stimulated for two short-term (subthreshold) periods (5-7 h) either sequentially or separated by an interval of rest. Naive lymph node T cells were able to integrate interrupted stimulation, even when the duration of rest was as long as 2 days. Furthermore, when short-term-stimulated T cells were separated by density, three populations were observed: low density blasts, intermediate density G, cells, and high density G,, cells. Low density cells progressed to division without further stimulation, whereas G, and G, cells remained undivided. However, after a period of rest, a second subthreshold stimulation caused the G, but not the G,, fraction to quickly proceed through the cell cycle. We conclude that noncycling T cells in the G, phase of the cell cycle remain in a state of readiness for prolonged periods of time, and may represent a population of memory-like effectors capable of responding rapidly to antigenic challenge.
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