期刊
ACTA TROPICA
卷 94, 期 1, 页码 55-59出版社
ELSEVIER
DOI: 10.1016/j.actatropica.2004.12.003
关键词
polymerase chain reactions; Leishmania braziliensis; mucosal leishmaniasis
We evaluated the use of polymerase chain reaction (PCR) for diagnosis of mucosal leishmaniasis (ML) in an endemic area in Acre, Brazil, where Leishmania braziliensis is present. Leishmania DNA was detected 34 of 35 cases, yielding a positivity rate of 97.1%, which was higher than the positivity rates for all of the other diagnostic methods studied, namely Montenegro skin test (MST), anti-Leishmania serological testing and microscopic examination of lesion biopsy specimens. These findings have led us to propose guidelines for the diagnosis of ML that use PCR as the principal method of parasitological confirmation of cases. (c) 2005 Elsevier B.V. All rights reserved.
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