期刊
RESEARCH IN VETERINARY SCIENCE
卷 78, 期 2, 页码 177-181出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2004.08.005
关键词
Fasciola hepatica; cattle; immunodiagnosis; ELISA
An ELISA was developed for the detection of Fasciola hepatica antibody in serum of cattle. The assay was applied to sera from 258 naturally infected cattle, 256 non-infected cattle and six calves experimentally infected with F hepatica. The diagnostic sensitivity and specificity of the ELISA test was 98% (95% confidence intervals, 96-100%) and 96% (95% confidence intervals, 93-98%) respectively at a cut-off value of 15% positivity. The results using sera from the experimentally infected calves showed that antibodies were first detected 2-4 weeks after infection. The ELISA test was also compared to the commercially available Bio-X bovine F. hepatica ELISA kit. A subset of 39 positive sera and 47 negative sera were selected from the samples used to evaluate the in-house test. The results indicated that the agreement between the two tests was almost perfect (k statistic = 0.82). (C) 2004 Elsevier Ltd. All rights reserved.
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