期刊
GENESIS
卷 41, 期 4, 页码 165-170出版社
WILEY
DOI: 10.1002/gene.20107
关键词
skeletal muscle alpha-actin; Cre/LoxP technology; Cre-ERT2 recombinase; tamoxifen; skeletal muscle
To generate temporally controlled targeted somatic mutations selectively and efficiently in skeletal muscles, we established a transgenic HSA-Cre-ERT2 mouse line in which the expression of the tamoxifen-dependent Cre-ERT2 recombinase is under the control of a large genomic DNA segment of the human skeletal muscle a-actin gene, contained in a P1-derived artificial chromosome. In this transgenic line Cre-ERT2 is selectively expressed in skeletal muscles, and Cre-ERT2-mediated alteration of LoxP flanked (floxed) target genes is skeletal muscle-specific and strictly tamoxifen-dependent. HSA-Cre-ERT2 mice should be of great value to analyze gene function in skeletal muscles, and to establish animal models of human skeletal muscle disorders. (c) 2005 Wiley-Liss, Inc.
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