期刊
EXPERT REVIEW OF PROTEOMICS
卷 2, 期 2, 页码 243-252出版社
TAYLOR & FRANCIS LTD
DOI: 10.1586/14789450.2.2.243
关键词
2D-DIGE; 2D-PAGE; fluorescence; functional proteomics; glycosylation; isotope-coded 2D maps; phophorylation; ProQ diamond; ProQ emerald; protein prefractionation
2D electrophoresis is currently the most widespread technique used for performing functional proteomics (i.e., the large-scale analysis of alterations in protein expression levels). Nevertheless, several limitations inherent to this technology have restricted the full potential of this protein differential display methodology for years. This has even led to the abandonment of 2D electrophoresis by several groups that switched to performing gel-free functional proteomics analyses based on liquid chromatography and mass spectrometry. Meanwhile, important recent advances in 2D electrophoresis, such as the introduction of fluorescent 2D difference gel electrophoresis and numerous protein prefractionation techniques, have thoroughly modernized 2D electrophoresis, making it again one of the preferred methods for the analysis of protein expression differences in many laboratories.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据