期刊
EUKARYOTIC CELL
卷 4, 期 4, 页码 685-693出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/EC.4.4.685-693.2005
关键词
-
资金
- NHLBI NIH HHS [R01 HL55949, R01 HL055949] Funding Source: Medline
- NIAID NIH HHS [R37 AI42747, R01 AI052303, T32 AI055397, R37 AI042747, R01 AI52303] Funding Source: Medline
The YPS3 gene of Histoplasma capsulatum encodes a protein that is both resident in the cell wall and also released into the culture medium. This protein is produced only during the pathogenic yeast phase of infection and is also expressed differently in H. capsulatum strains that differ in virulence. We investigated the cellular localization of Yps3p. We demonstrated that the cell wall fraction of Yps3p was surface localized in restriction fragment length polymorphism class 2 strains. We also established that Yps3p released into the G217B culture supernatant binds to the surface of strains that do not naturally express the protein. This binding was saturable and occurred within 5 min of exposure and occurred similarly with live and heat-killed H. capsulatum. Flow cytometric analysis of H. capsulatum after enzymatic treatments was consistent with Yps3p binding to chitin, a carbohydrate polymer that is a component of fungal cell walls. Polysaccharide binding assays demonstrated that chitin but not cellulose binds to and extracts Yps3p from culture supernatants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据