Calcium phosphate - mediated transfection of eukaryotic cells

The uptake of DNA is markedly enhanced when the nucleic acid is presented as a coprecipitate of calcium phosphate and DNA(1). The insoluble precipitate attaches to the cell surface and is brought into the cells by endocytosis. Since the publication of the original method(1), increases in efficiency have been achieved by including additional steps such as glycerol shock(2) and/or chloroquine treatment(3). This protocol is a modified version of a published method(4), in which calcium phosphate-based transfection methods for Chinese hamster ovary cells and the 293 Line of human embryonic kidney cells were rigorously optimized. The protocol is easily adapted for use with other types of cells, both adherent and nonadherent.