期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 102, 期 14, 页码 5150-5155出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0408995102
关键词
autoimmunity; tolerance; metabolism; hormones
We analyzed the serum and cerebrospinal fluid (CSF) leptin secretion and the interaction between serum leptin and CD4(+)CD25(+) regulatory T cells (T-Regs) in naive-to-therapy relapsing-remitting multiple sclerosis (RRMS) patients. Leptin production was significantly increased in both serum and CSF of RRMS patients and correlated with IFN-gamma secretion in the CSF. T cell lines against human myelin basic protein (hMBP) produced immunoreactive leptin and up-regulated the expression of the leptin receptor (ObR) after activation with hMBP. Treatment with either anti-leptin or anti-leptin-receptor neutralizing antibodies inhibited in vitro proliferation in response to hMBP. Interestingly, in the RRMS patients, an inverse correlation between serum leptin and percentage of circulating TRegs was also observed. To better analyze the finding, we enumerated T-Regs in leptin-deficient (ob/ob) and leptin-receptor-deficient (db/db) mice and observed the significant increase in T-Regs. Moreover, treatment of WT mice with soluble ObR fusion protein (ObR:Fc) increased the percentage of T-Regs and ameliorated the clinical course and progression of disease in proteolipid protein peptide (PLP139-151)-induced relapsing-experimental autoimmune encephalomyelitis (R-EAE), an animal model of RRMS. These findings show an inverse relationship between leptin secretion and the frequency of TRegs in RRMS and may have implications for the pathogenesis of and therapy for multiple sclerosis.
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