Assimilation and biosynthesis of lipids in Arctic Calanus species based on feeding experiments with a 13C labelled diatom

The dominant Arctic Ocean and North Atlantic copepods Calanus hyperboreus, Calanus glacialis, and Calanus finmarchicus were collected in the Greenland Sea and fed C-13 labelled diatom Thalassiosira weissflogii to follow the transfer and assimilation of carbon, lipid, and individual fatty acids and alcohols. The diatom was grown with 13C for 3 to 5 days and fed then to the copepods. During the feeding period of 14 days, total carbon increased in the copepodite stages V of C hyperboreus and C. finmarchicus, whereas carbon remained almost constant in C glacialis females. However, total lipid increased in all species and stages. Highest lipid accumulation occurred in C hyperboreus in which nearly all lipids were exchanged already after I I days of feeding. In the other species lipid accumulation made up between 22% (C. finmarchicus) and 45% of total lipid (C glacialis). The proportion of wax esters was high ranging from 76% of total lipid in C glacialis to 92% in C. finmarchicus. The fatty acid composition of the alga was dominated by 16:1(n-7), 16:0, 20:5(n-3), and 22:6(n-3). The composition of the copepods was similar because of feeding already on diatoms in the field. In addition, the monounsaturated fatty acids and alcohols, 20: 1 (n-9) and 22: 1 (n- 11), were major components of the copepod lipids. During the feeding period the highest 13C labelling was always found in the C-16 polyunsaturated fatty acids and in the 16:1(n-7) alcohol. Because these components occurred only in trace amounts in the copepods they totally originated from the diet explaining the high labelling. It is noteworthy that the 16: 1 (n-7) alcohol originated only from the corresponding dietary and not from the abundant internal fatty acid. The long-chain monounsaturated fatty acids and alcohols, 20: 1 (n-9) and 22: 1 (n- 11), are not existent in phytoplankton and have to be produced de novo. They were less labelled in the smaller species but highly C-13 enriched in C. hyperboreus. Although dietary fatty acids were generally retained by the copepods it seems that fatty acids or even lipids were selectively accumulated and turned over due to bodily requirements, and thus, essential polyunsaturated fatty acids were preferentially retained. During feeding mixing, accumulation, and exchange of internal and dietary fatty acids and alcohols occurred as well as utilisation of lipids from both sources for metabolic requirements. The differences in lipid assimilation fit to the different life strategies of the copepods. (c) 2004 Elsevier B.V All rights reserved.