期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 280, 期 14, 页码 14189-14202出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M413660200
关键词
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资金
- NIGMS NIH HHS [R01GM64350, R01GM49164] Funding Source: Medline
Protein ubiquitination and subsequent degradation by the proteasome are important mechanisms regulating cell cycle, growth and differentiation, and apoptosis. Recent studies in cancer therapy suggest that drugs that disrupt the ubiquitin/ proteasome pathway induce apoptosis and sensitize malignant cells and tumors to conventional chemotherapy. In this study we addressed the role of phosphorylation of the alpha- subunit eukaryotic initiation factor- 2 ( eIF2), and its attendant regulation of gene expression, in the cellular stress response to proteasome inhibition. Phosphorylation of eIF2 alpha in mouse embryo fibroblast ( MEF) cells subjected to proteasome inhibition leads to a significant reduction in protein synthesis, concomitant with induced expression of the bZIP transcription regulator, ATF4, and its target gene CHOP/ GADD153. The primary eIF2 alpha kinase activated by exposure of these fibroblast cells to proteasome inhibition is GCN2 ( EIF2AK4), which has a central role in the recognition of cytoplasmic stress signals. Endoplasmic reticulum ( ER) stress is not effectively induced in MEF cells subjected to proteasome inhibition, with minimal activation of the ER stress sensory proteins, eIF2 alpha kinase PEK ( PERK/ EIF2AK3), IRE1 protein kinase and the transcription regulator ATF6 following up to 6 h of proteasome inhibitor treatment. Loss of eIF2 alpha phosphorylation thwarts caspase activation and delays apoptosis. Central to this pro- apoptotic function of eIF2 alpha kinases during proteasome inhibition is the transcriptional regulator CHOP, as deletion of CHOP in MEF cells impedes apoptosis. We conclude that eIF2 alpha kinases are integral to cellular stress pathways induced by proteasome inhibitors, and may be central to the efficacy of anticancer drugs that target the ubiquitin/ proteasome pathway.
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