期刊
VACCINE
卷 23, 期 20, 页码 2580-2590出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2004.11.035
关键词
Salmonella; live recombinant vaccine; genetic engineering; heterologous antigen expression
Salmonella enterica serovar Typhimurium is a versatile host organism for the generation of recombinant live vaccines for mucosal immunization. We investigated the performance of an intracellular-activated promoter for expression of heterologous antigens by Salmonella carrier strains. We observed that intracellular expressed antigens were more efficient in elicitation of humoral and cellular immune responses than constitutively expressed antigens. Furthermore, the intracellular-activated promoter was more efficient in combination with a carrier strain deficient in SPI2 gene sseC than with the conventional aroA strain. We propose that intracellular-activated promoters will be useful tools for construction of efficient recombinant live vaccines. (c) 2004 Elsevier Ltd. All rights reserved.
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