期刊
ANALYTICA CHIMICA ACTA
卷 535, 期 1-2, 页码 219-225出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2004.12.009
关键词
carbaryl; flow-through ELISA; dipstick ELISA; on-site determination
Membrane-based competitive enzyme immunoassays in flow-through and dipstick format for the rapid detection of carbaryl were developed. A nylon membrane was coated with anti-carbaryl antibody and a carbaryl-horseradish peroxidase (HRP) conjugate was used as the labeled antigen for competitive assay of carbaryl. Both tests had visual detection limits of 10 mu g L-1, sensitive enough to meet the requirements of regulatory agencies as a practical screening tool. Matrix interference was eliminated by appropriate dilution of sample extracts with buffer. With assay times of 5 min (flow-through) and 15 min (dipstick), these rapid tests can be useful as a quality control tool and can be used to qualitatively detect carbaryl. (c) 2004 Elsevier B.V. All rights reserved.
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