期刊
IUBMB LIFE
卷 67, 期 11, 页码 828-836出版社
WILEY
DOI: 10.1002/iub.1439
关键词
oxyresveratrol; tyrosinase; alternative substrate; hydrogen peroxide; kinetic characterization
资金
- Fundacion Seneca, CARM, Murcia, Spain [19545/PI/14, 19304/PI/14, 19240/PI/14]
- MINECO, Madrid [SAF2013-48375-C2-1-R, CTQ2014-56887-P]
- University of Murcia, Murcia [UMU15452, UMU17766]
- MEC-FPU fellowship [AP2010-4300]
Oxyresveratrol is a stilbenoid described as a powerful inhibitor of tyrosinase and proposed as skin-whitening and anti-browning agent. However, the enzyme is capable of acting on it, considering it as a substrate, as it has been proved in the case of its analogous resveratrol. Tyrosinase hydroxylates the oxyresveratrol to an o-diphenol and oxidizes the latter to an o-quinone, which finally isomerizes to p-quinone. For these reactions to take place the presence of the E-ox (oxy-tyrosinase) form is necessary. The kinetic analysis of the proposed mechanism has allowed the kinetic characterization of this molecule as a substrate of tyrosinase, affording a catalytic constant of 5.39 +/- 0.21 sec(-1) and a Michaelis constant of 8.65 +/- 0.73 mu M. (C) 2015 IUBMB Life.
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