期刊
BIOSENSORS & BIOELECTRONICS
卷 20, 期 10, 页码 2120-2125出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2004.08.023
关键词
cell manipulation; gene transfer; AFM; force-distance curve; avidin-biotin unbinding force
We developed a new low invasive cell manipulation and gene or molecule transfer system in a single living cell by using an atomic force microscope (AFM) and ultra thin needle, a nanoneedle. DNA was immobilized on the surface of the nanoneedle by covalent bonding and avidin-biotin affinity binding. Immobilization of DNA on the nanoneedle was confirmed by measuring the unbinding force between avidin and biotin. The DNA-immobilized nanoneedle was successfully inserted into HEK293 cells. Though TO-PRO-3 iodide staining experiments using confocal microscopy, we observed the immobilized DNA on the surface of the nanoneedle, which was retained after 10 times insertions to and evacuations from a living cell. (c) 2004 Elsevier B.V. All rights reserved.
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