期刊
JOURNAL OF CELL SCIENCE
卷 118, 期 8, 页码 1641-1649出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.02276
关键词
endocytic recycling compartment (ERC); endocytosis; synaptotagmin; protein kinase C; RBL-2H3 mast cells
类别
Neuronal and non-neuronal tissues show distinctly different intracellular localization of synaptotagmin (Syt) homologues. Therefore, cell type-specific mechanisms are likely to direct Syt homologues to their final cellular destinations. Syt IX localizes to dense core vesicles in PC12 cells. However, in the rat basophilic leukemia (RBL-2H3) mast cell line, as well as in CHO cells, Syt IX is localized at the endocytic recycling compartment (ERC). We show that targeting of Syt IX to the ERC involves constitutive trafficking to the plasma membrane followed by internalization and transport to the ERC. We further show that internalization from the plasma membrane and delivery to the ERC are dependent on phosphorylation by Ca2+-dependent protein kinase C alpha or beta. As such, correct targeting of Syt IX is facilitated by the phorbol ester TPA but prevented by the cPKC inhibitor Go 6976.
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