Acute biochemical effects of La(NO3)3 on liver and kidney tissues by magic-angle spinning 1H nuclear magnetic resonance spectroscopy and pattern recognition

High-resolution magic-angle spinning (MAS) H-1 nuclear magnetic resonance (NMR) spectroscopic and pattcrn recognition (PR) based methods have been applied to studies on the acute biochemical effects of La(NO3)(3) on rats. Male Wistar rats were treated with various doses of La(NO3)(3) (2, 10, and 50 rng/kg body weight), and MAS H-1 NMR spectra of their intact liver and kidney tissues were analyzed using principal components analysis to extract metabolic information. The biochemical effects of La(NO3)(3) were characterized by the increase of triglyceride and bile acid and the decrease of glycogen in liver tissue, together with a slight elevation of triglyceride level in kidney tissue. The target lesion of La(NO3)(3) to liver was found by MAS NMR-PR methods. This study illustrated the power of the combination of MAS H-1 NMR and pattern recognition for the analysis of biochemical effects of rare earths. (c) 2005 Elsevier Inc. All rights reserved.