期刊
ANALYTICAL BIOCHEMISTRY
卷 340, 期 1, 页码 80-88出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2005.02.007
关键词
aryl sulfatases; continuous; fluorescence assay; 6.8-difluoro-4-methylumbelliferyl sulfate
The most common fluorogenic substrate for assaying aryl sulfatases (ARSs) is 4-methylumbelliferyl sulfate (MUS). However, ARSs operate optimally at pH values that are less than the pK(a) (7.8) of the reaction product of MUS, 4-methylumbelliferone (4-MU). Thus, a major disadvantage of this assay is that it is usually run in a discontinuous mode due to the need for basification of the reaction mixture to achieve complete ionization of the phenolic products and maximum fluorescence. To circumvent this problem, 6,8-difluoro-4-methylumbelliferyl sulfate (DiFMUS) was prepared and examined as a substrate for ARSs. The product of the reaction is 6,8-difluoro-4-methylumbelliferone, a known coumarin with fluorescent properties equal to those of 4-MU, and has a pK(a) of 4.9. This allowed for the continuous assaying of human placental ARSs A, 13, and C, which operate optimally between pH 5.0 and pH 7.0. Furthermore, DiFMUS exhibited a lower K-m (up to 20-fold) for the ARSs than did MUS; for ARSA and ARSB, it exhibited a greater V-max than did MUS. This substrate should have considerable utility for the continuous assay of ARS activity. (c) 2005 Elsevier Inc. All rights reserved.
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