期刊
JOURNAL OF PROTEOME RESEARCH
卷 4, 期 3, 页码 982-991出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr050054d
关键词
proteomics; membrane proteins; gradient gels; third separation; peptides
Here we have addressed common issues of resolution in two-dimensional polyacrylamide gel electrophoresis (2DE) experiments including proteins 'stacked' at pH extremes, unresolved peptides migrating at the front of separation, and areas of the 2D gel obscured by high abundance proteins. Postfractionation, by selective application of well-established electrophoretic separations immediately following standard 2DE, yields markedly improved resolution in these traditional problem areas using no more specialized equipment or techniques than SDS-PAGE itself.
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