期刊
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
卷 88, 期 1, 页码 173-189出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pbiomolbio.2004.01.007
关键词
whole genome amplification; PEP; DOP-PCR; SCOMP; SDA; TLAD
The availability of large amounts of genomic DNA is of critical importance for many of the molecular biology assays used in the analysis of human disease. However, since the amount of patient tissue available is often limited and as particular foci of interest may consist of only a few hundred cells. the yield of DNA is often insufficient for extensive analysis. To address this problem, several whole genome amplification (WGA) methodologies have been developed. Initial WGA approaches were based on the polymerase chain reaction (PCR). However, recent reports have described the use of non-PCR-based linear amplification protocols for WGA. Using these methods, it is possible to generate microgram quantites of DNA starting with as little as 1 ma of genomic DNA. This review will provide an overview of WGA approaches and summarize some of the uses for amplified DNA in various high-throughput genetic applications. (C) 2004 Elsevier Ltd. All rights reserved.
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