期刊
LUNG CANCER
卷 48, 期 2, 页码 223-231出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.lungcan.2004.11.011
关键词
SCLC; TAM; CHI3L1; invasion; HC GP-39
YKL-40 is a 40 kDa protein with possible involvement in tissue remodeling, cell proliferation and angiogenesis. Elevated serum YKL-40 Levels in patients with metastatic cancers (including small cell lung cancer (SCLC)) are associated with poor prognosis. The aim of this study was to identify the cellular source of YKL-40 in SCLC patient biopsies and in a panel of 20 human SCLC lines cultured in vitro and in vivo in nude mice. In general, the SCLC cell lines had no or very limited (human) YKL-40 expression, whereas, by RT-PCR a pronounced murine (i.e., stromal) YKL-40 expression was present in all tumors. YKL-40 mRNA transcripts were detected by in situ hybridization in 9 of 10 biopsies from SCLC patients, and in each case the signal was localized in the peritumoral stroma in cells of typical macrophage morphology (confirmed by a CD68 macrophage specific stain). No YKL-40 mRNA expression was found in the cancer cells, in macrophages infiltrating the solid tumor areas, or in nonmalignant tissue. In conclusion, the predominant source of elevated serum YKL-40 in SCLC is peritumoral macrophages. (c) 2004 Elsevier Ireland Ltd. All rights reserved.
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