期刊
EUKARYOTIC CELL
卷 4, 期 5, 页码 861-866出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/EC.4.5.861-866.2005
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资金
- NIGMS NIH HHS [R01 GM034095, R37 GM034095, GM34095] Funding Source: Medline
In Saccharomyces cerevisiae, Snf1 protein kinase of the Snf1/AMP-activated protein kinase family is required for growth on nonfermentable carbon sources and nonpreferred sugars. Three kinases, Pak1, Elm1, and Tos3, activate Sun by phosphorylation of its activation-loop threonine, and the absence of all three causes the Snf(-) phenotype. No phenotype has previously been reported for the tos3 Delta single mutation. We show here that, when cells are grown on glycerol-ethanol, tos3 Delta reduces growth rate, Snf1 catalytic activity, and activation of the Snf1-dependent carbon source-responsive element (CSRE) in the promoters of gluconeogenic genes. In contrast, tos3 Delta did not significantly affect Sun catalytic activity or CSRE function during abrupt glucose depletion, indicating that Tos3 has a more substantial role in activating Snf1 protein kinase during growth on a nonfermentable carbon source than during acute carbon stress. We also report that Tos3 is localized in the cytosol during growth in either glucose or glycerol-ethanol. These findings lend support to the idea that the Snf1 protein kinase kinases make different contributions to cellular regulation under different growth conditions.
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