期刊
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
卷 34, 期 3, 页码 255-261出版社
SPRINGER
DOI: 10.1007/s00249-004-0447-7
关键词
actin meshwork viscoelasticity; myosin; microrheology; optical tweezers; blebbistatin
类别
We have determined the microrheological response of the actin meshwork for individual cells. We applied oscillating forces with an optical tweezer to a micrometric bead specifically bound to the actin meshwork of C2 myoblasts, and measured the amplitude and phase shift of the induced cell deformation. For a non-perturbed single cell, we have shown that the elastic and loss moduli G' and G '' behave as power laws f(alpha) and f(beta) of the frequency f (0.01< f < 50 Hz), alpha and beta being in the range 0.15 - 0.35. This demonstrates that the dissipation mechanisms in a single cell involve a broad and continuous distribution of relaxation times. After adding blebbistatin, an inhibitor of myosin II activity, the exponent of G' decreases to about 0.10, and G '' becomes roughly constant for 0.01< f< 10 Hz. The actin meshwork appears less rigid and less dissipative than in the control experiment. This is consistent with an inhibition of ATPase and reduction of the gliding mobility of myosin II on actin. laments. In this frequency range, the actomyosin activity appears as an essential mechanism allowing the cell to adapt to an external mechanical stress.
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