Removal of contaminating DNA from commercial nucleic acid extraction kit reagents

Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was effective in removing this reagent-derived contaminating DNA while the sensitivity of the assay was maintained. (C) 2004 Elsevier B.V. All rights reserved.