期刊
RNA
卷 11, 期 5, 页码 774-784出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.7286705
关键词
covariance model; natural aptamer; methenyltetrahydrofolate synthetase; noncoding RNA; stationary phase
资金
- NHGRI NIH HHS [T32 HG000035, R01 HG02602, HG-00035, R01 HG002602] Funding Source: Medline
- NIGMS NIH HHS [GM 068819, R01 GM068819] Funding Source: Medline
6S RNA is an abundant noncoding RNA in Escherichia coli that binds to sigma(70) RNA polymerase holoenzyme to globally regulate gene expression in response to the shift from exponential growth to stationary phase. We have computationally identified > 100 new 6S RNA homologs in diverse eubacterial lineages. Two abundant Bacillus subtilis RNAs of unknown function (BsrA and BsrB) and cyanobacterial 6Sa RNAs are now recognized as 6S homologs. Structural probing of E. coli 6S RNA and a B. subtilis homolog supports a common secondary structure derived from comparative sequence analysis. The conserved features of 6S RNA suggest that it binds RNA polymerase by mimicking the structure of DNA template in an open promoter complex. Interestingly, the two B. subtilis 6S RNAs are discoordinately expressed during growth, and many proteobacterial 6S RNAs could be cotranscribed with downstream homologs of the E. coli ygfA gene encoding a putative methenyltetrahydrofolate synthetase. The prevalence and robust expression of 6S RNAs emphasize their critical role in bacterial adaptation.
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