期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 25, 期 10, 页码 3896-3905出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.25.10.3896-3905.2005
关键词
-
资金
- NIAID NIH HHS [U19 AI056900] Funding Source: Medline
- PHS HHS [P01 A156900] Funding Source: Medline
RNA interference (RNAi) is a naturally occurring posttranscriptional gene-silencing mechanism that has been adapted as a genetic tool for loss-of-function studies of a variety of organisms. It is more widely applicable than classical gene targeting and allows for the simultaneous inactivation of several homologous genes with a single transgene. Recently, RNAi has been used for conditional and conventional gene inactivation in mice. Unlike gene targeting, RNAi is a dynamic process, and its efficiency may vary both between cell types and throughout development. Here we demonstrate that RNAi can be used to target three separately encoded isoforms of the bcl-2 family gene bfl-1/A1 in a conditional manner in mice. The extent of gene inactivation varies between different cell types and is least efficient in mature lymphocytes. Our data suggest that RNAi is affected by factors beyond small interfering RNA-mRNA stoichiometry.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据