期刊
JOURNAL OF MEDICAL MICROBIOLOGY
卷 54, 期 5, 页码 453-455出版社
MICROBIOLOGY SOC
DOI: 10.1099/jmm.0.45836-0
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Detection of the conserved capsule gene bexA is used to distinguish capsulate from non-capsulate Haemophilus influenzae. While developing a real-time PCR assay to detect bexA, it was found that bexA probes produced a detectable signal for H. influenzae types a to d, but failed to do so for H. influenzae types e and f. Sequencing revealed differences compared with H. influenzae types a to d within probe binding sites. To prevent misclassification of strains as non-capsulate, assays must detect all capsular types.
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