期刊
PLANT CELL REPORTS
卷 24, 期 2, 页码 86-94出版社
SPRINGER
DOI: 10.1007/s00299-004-0909-5
关键词
marker-free transformation; rice; Cre; loxP; DNA recombination
Removal of a selectable marker gene from genetically modified (GM) crops alleviates the risk of its release into the environment and hastens the public acceptance of GM crops. Here we report the production of marker-free transgenic rice by using a chemically regulated, Cre/loxP-mediated site-specific DNA recombination in a single transformation. Among 86 independent transgenic lines, ten were found to be marker-free in the T-0 generation and an additional 17 lines segregated marker-free transgenic plants in the T-1 generation. Molecular and genetic analyses indicated that the DNA recombination and excision in transgenic rice were precise and the marker-free recombinant T-DNA was stable and heritable.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据