期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 25, 期 10, 页码 3923-3933出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.25.10.3923-3933.2005
关键词
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Epigenetic inactivation of the RASSFIA tumor suppressor by CpG island methylation was frequently detected in cancer. However, the mechanisms of this aberrant DNA methylation are unknown. In the RASSFIA promoter, we characterized four Sp1 sites, which are frequently methylated in cancer. We examined the functional relationship between DNA methylation, histone modification, Sp1 binding, and RASSFIA expression in proliferating human mammary epithelial cells. With increasing passages, the transcription of RASSFIA was dramatically silenced. This inactivation was associated with deacetylation and lysine 9 trimethylation of histone H3 and an impaired binding of Sp1 at the RASSFIA promoter. In mammary epithelial cells that had overcome a stress-associated senescence barrier, a spreading of DNA methylation in the CpG island promoter was observed. When the RASSFIA-silenced cells were treated with inhibitors of DNA methyltransferase and histone deacetylase, binding of Sp1 and expression of RASSFIA reoccurred. In summary, we observed that histone H3 deacetylation and H3 lysine 9 trimethylation occur in the same time window as gene inactivation and precede DNA methylation. Our data suggest that in epithelial cells, histone inactivation may trigger de novo DNA methylation of the RASSFIA promoter and this system may serve as a model for CpG island inactivation of tumor suppressor genes.
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