期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 69, 期 5, 页码 1036-1039出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.69.1036
关键词
Bacillus subtilis genome; transformation; inositol; phleomycin; pBR322
The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据