Preparation of deuterated abscisic acid metabolites for use in mass spectrometry and feeding studies

Four deuterium-labelled ABA catabolites of high isotopic purity were prepared for hormone profiling studies using HPLC-ESI-MS-MS. The catabolites, namely [7',7',7'-d(3)]-PA, [7',7',7'-d(3)-]-DPA, [5,8,8',8-d(4)]-7'-hydroxyABA and [4,5,8',8',8-d(5)]-ABA glucose ester were prepared either by biotransformation or chemical synthesis starting with labelled ABA. The labelled compounds are employed as internal standards for plant hormone profiling by liquid chromatography-electrospray-tandem mass spectrometry. Copyright (c) 2005 John Wiley & Sons, Ltd.