期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 35, 期 5, 页码 1530-1538出版社
WILEY
DOI: 10.1002/eji.200425895
关键词
gp49B1; arthritis; inflammation; neutrophils; immunoreceptor; tyrosine-based inhibitory motif
类别
资金
- NHLBI NIH HHS [HL-36110] Funding Source: Medline
- NIAID NIH HHS [AI-31599, AI-41144] Funding Source: Medline
Mice with a disrupted gp49B gene, which encodes gp49B1 that is expressed on certain hematopoietic cells and has two immunoreceptor tyrosine-based inhibitory motifs (ITIM), exhibit augmented Fc epsilon RI-initiated mast cell degranulation and resultant tissue edema. gp49B1 -deficient (gp49B(-/-) ) mice also exhibit exaggerated lipopolysaccharide (LPS)-induced intravascular neutrophil aggregation leading to cutaneous microangiopathy. To determine whether gp49B(-/-) mice exhibit elevated cytokine and chemokine levels leading to pathologic inflammation, we quantified clinical and morphologic parameters of arthritis and tissue levels of contributory mediators in gp49B(-/-) and gp49B1 -sufficient (gp49B(+/+)) mice injected with anti-type 11 collagen monoclonal antibody (mAb) and LPS. Clinical scores for joint swelling and histological assessments of synovial thickness and cartilage matrix depletion at day 7 were significantly 2.3- to 2.5-fold greater and were more prolonged in gp49B(-/-) mice. At day 5, the amounts of IL-1 beta, macrophage inflammatory protein (MIP)-1a, and MIP-2 were 2.1-, 2.5-, and 12-fold greater in joint extracts from gp49B(-/-) mice. A significant 2.7-fold more neutrophils infiltrated the synovium of gp49B(-/-) mice at day 7, and neutrophilia persisted with the delayed resolution of the synovitis. mAb-mediated depletion of neutrophils prevented the synovitis in both strains. Thus, gp49B1 counter-regulates the cytokine and chemokine induction and attendant neutrophilia that are all essential for synovitis and cartilage matrix depletion.
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