期刊
TISSUE ENGINEERING
卷 11, 期 5-6, 页码 825-834出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.2005.11.825
关键词
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Alignment of bone cells and collagen matrix is closely related to the anisotropic mechanical properties of bone. Intact scaffolds that promote osteoblast differentiation and mineralization in the preferred direction offer promise in the generation of biomimetic bone tissue. In this study, we examined the alignment of osteoblast-like cells and collagen fibers guided by nanogrooves. Nanoscale groove-ridge patterns (similar to 300 nm in periodicity, 60-70 nm in depth) on the surface of polystyrene ( PS) were made by polarized Nd:YAG laser irradiation, at a wavelength of 266 nm. The influence of such nanoscale features on the orientation and alignment of cells and their mineralized collagen matrix was investigated, using rabbit mesenchymal stem cell (MSC)-derived osteoblast-like cells. The cells and actin stress fibers were aligned and elongated along the direction of the nanogrooves. In addition, the alignment of collagen matrix was also influenced by underlying nanogrooves. The results suggested that nanoscale fibrous cues in the longitudinal direction might contribute to the aligned formation of bone tissue. This may provide an effective approach for constructing biomimetic bone tissue.
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