期刊
DEVELOPMENTAL DYNAMICS
卷 233, 期 1, 页码 105-114出版社
WILEY
DOI: 10.1002/dvdy.20305
关键词
zebrafish; epiboly; radial intercalation; eomesodermin; mtx2
资金
- NCRR NIH HHS [P40 RR12546, P40 RR012546] Funding Source: Medline
- NIGMS NIH HHS [R01 GM067714-04, R01 GM067714] Funding Source: Medline
The T-box gene eomesodermin (eomes) has been implicated in mesoderm specification and patterning in both zebrafish and frog. Here, we describe an additional function for eomes in the control of morphogenesis. Epiboly, the spreading and thinning of an epithelial cell sheet, is a central component of gastrulation in many species; however, despite its importance, little is known about its molecular control. Here, we show that repression of eomes function in the zebrafish embryo dramatically inhibits epiboly movements. We also show that eomes regulates the expression of a zygotic homeobox transcription factor mtx2. Gene knockdown of mtx2 using antisense morpholino oligonucleotides, likewise, leads to an inhibition of epiboly; moreover, we show that knockdown of mtx2 function in the extraembryonic yolk syncytial layer only is sufficient to cause epiboly defects. Thus, we have identified two components in a molecular pathway controlling epiboly and show that interactions between deep layer cells of the embryo proper and extraembryonic tissues contribute in a coordinated manner to different aspects of epiboly movements. (c) 2005 Wiley-Liss, Inc.
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