Simple method for the simultaneous isolation and determination of fumonisin B1 and its metabolite aminopentol-1 in swine liver by liquid chromatography-fluorescence detection

An analytical method based on high-performance liquid chromatography (HPLC) combined with fluorescence detection (FL) has been developed for the simultaneous determination of fumonisin B, (FBI) and its totally hydrolized metabolite aminopentol-1 (AP(1)) in pig liver. The sample preparation is based on a single solid phase extraction (SPE). o-Phthalaldehyde (OPA) was used for pre-column derivatization before the programmed reversed-phase analysis on phenylhexyl column. The developed method shows good repeatibility for inter- and intra-day precision as well as adequate linearity of calibration curves (r(2) was 0.9855 for FB1 and 0.9831 for AP(1)). Average recoveries from the matrix were 93.6% for FB1 and 95.3% for AP(1). The limit of quantification (LOQ) in swine liver was 75 mu g/kg for FB1 and 42 mu g/kg for AP(1). (c) 2005 Elsevier B.V. All rights reserved.