期刊
APPLIED ENTOMOLOGY AND ZOOLOGY
卷 44, 期 4, 页码 595-601出版社
JAPAN SOC APPL ENTOMOL ZOOL
DOI: 10.1303/aez.2009.595
关键词
Plant-herbivore interactions; anti-nutritive plant defense; coevolution; essential amino acids; chemical ecology
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT)
- Pioneer Research Project Funds
- Ministry of Agriculture, Forestry and Fisheries of Japan (MAFF)
The leaves of the privet tree, Ligustrum obtusifolium (Oleaceae), retain a strong lysine-decreasing activity caused by enzymatically-activated oleuropein, an iridoid glycoside. Protein treated with this activity become innutritive to insects because of the loss of lysine. We previously found that several privet specialist caterpillars secrete high concentrations of glycine in their digestive juices. Previous in vitro experiments showed that glycine inhibits lysine-decreasing activity of oleuropein. Therefore, we hypothesized that the lysine-decreasing activity acts to defend privet tree against herbivores and that glycine secretion by insects is an adaptive trait to counter the privet defense. In the present study, we aimed to examine whether these assumptions hold true under physiological conditions, and performed in vivo bioassays and physiological analyses using the Eri silkworm, Samia ricini (Saturniidae), a non-privet specialist. Significant decreases in larval growth and lysine concentration in the midgut lumen were observed when larvae were fed intact privet leaves compared to when they were fed heat inactivated privet leaves. These decreases were inhibited when larvae were fed intact privet leaves together with glycine, indicating that the privet defense with oleuropein and the specialist adaptation with glycine do function under physiological conditions. This study thus provides a rare view into the detailed physiological impacts of anti-nutritive plant defense and insect physiological adaptation in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据