期刊
BLOOD
卷 105, 期 10, 页码 3862-3870出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2004-09-3611
关键词
-
类别
资金
- NHLBI NIH HHS [HL 48834, R01 HL71800-01, R01 HL65169] Funding Source: Medline
In this report, we have defined the stage at which SO functions in the establishment of the hematopoietic system and provide evidence that its primary role is in the generation of the hematopoietic lineages from a progenitor called the blast colony-forming cell (BL-CFC), a cell considered to be the in vitro equivalent of the hemangioblast. Using an embryonic stem (ES) cell line in which lacZcDNA has been targeted to the Scl locus, we show that most of the BL-CFCs are detected in the SCL/lacZ(-) population, indicating that this progenitor does not express Scl In the blast colony assay, Scl(-/-)cells initiate colony growth but are unable to generate endothelial and hematopoletic progeny and thus form colonies consisting of vascular smooth muscle cells only. The capacity to give rise to blast colonies can be rescued by retroviral transduction of a wild-type Scl gene into Scl(-/-) FLK-1(+) cells, suggesting that the BL-CFC is generated in this population. Finally, we show that Sci(-/-)endothelial cells display a growth deficiency in monolayer cultures that can be partially overcome by maintaining this population as 3-dimensional aggregates indicating that specific cellular interactions are required for maintenance of the Sci(-/-)endothelial lineage in vitro. (c) 2005 by The American Society of Hematology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据