期刊
ANALYTICAL BIOCHEMISTRY
卷 340, 期 2, 页码 341-351出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2005.02.015
关键词
heterotrimeric G-protein; regulator of G-protein signaling; RGS; GTPase accelerating protein; GAP; BODIPY; fluorescene
资金
- NIGMS NIH HHS [P01 GM065533, R01 GM062338] Funding Source: Medline
Diverse extracellular signals regulate seven transmembrane-spanning receptors to modulate cellular physiology. These receptors signal primarily through activation of heterotrimeric guanine nucleotide bindin proteins (G proteins). A major determinant of heterotrimeric G protein signaling in vivo and in vitro is the intrinsic GTPase activity of the G alpha subunit. RGS (regulator of G protein signaling) domain-containing proteins are GTPase accelerating proteins specific for G alpha subunits. In this article, we describe the use of the ribose-conjugated fluorescent guanine nucleotide analog BODIPYFL-GTP as a spectroscopic probe to measure intrinsic and RGS protein-catalyzed nucleotide hydrolysis by G alpha(o). BODIPYFL-GTP bound to G alpha(o) exhibits a 200% increase in fluorescence quantum yield. Hydrolysis of BODIPYFL-GTP to BODIPYFL-GDP reduces the quantum yield to 27% above its unbound value. We demonstrate that BODIPYFL-GTP can be used as a rapid real-time probe for measuring RGS domain-catalyzed GTP hydrolysis by G alpha(o). We demonstrate the effectiveness of this assay in the analysis of loss-of-function point mutants of both G alpha(o) and RGS12. This assay should be useful in screening for and analyzing RGS protein inhibitory compounds. (c) 2005 Elsevier Inc. All rights reserved.
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