期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 102, 期 20, 页码 7157-7162出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0409455102
关键词
molecular screening
资金
- NCRR NIH HHS [RR07707, P41 RR007707] Funding Source: Medline
- NIEHS NIH HHS [P30 ES000210] Funding Source: Medline
- NIGMS NIH HHS [R1GM62957A, R01 GM062957] Funding Source: Medline
The fundamental question of how sequence defines conformation is explicitly answered if the structures of all possible sequences of a macromolecule are determined. We present here a crystallo-graphic screen of all permutations of the inverted repeat DNA sequence d(CCnnnN(6)N(7)N(8)GG), where N-6, N-7, and N-8 are any of the four naturally occurring nucleotides. At this point, 63 of the 64 possible permutations have been crystallized from a defined set of solutions. When combined with previous work, we have assembled a data set of 37 single-crystal structures from 29 of the sequences in this motif, representing three structural classes of DNA (B-DNA, A-DNA, and four-stranded Holliday junctions). This data set includes a unique set of amphimorphic sequence, those that crystallize in two different conformations and serve to bridge the three structural phases. We have thus constructed a map of DNA structures that can be walked through in single nucleotide steps. Finally, the resulting data set allows us to dissect in detail the stabilization of and conformational variations within structural classes and identify significant conformational deviations within a particular structural class that result from sequence rather than crystal or crystallization effects.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据