期刊
BIOMOLECULAR ENGINEERING
卷 22, 期 1-3, 页码 57-61出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bioeng.2004.11.002
关键词
protein engineering; directed evolution; random mutagenesis; cassette mutagenesis; genetic libraries
The creation of protein libraries by random mutagenesis and cassette mutagenesis has proven to be a successful method of protein engineering. Appropriate statistical analysis is important for the proper construction of these libraries and even more important for the interpretation of data from these libraries. We present simple mathematical expressions useful in the creation and evaluation of such libraries. These equations are useful in estimating the distribution of mutations, the degeneracy of the library and the frequency of a particular clone in the library. In addition, general equations addressing the probability that a particular clone is in a library, the probability that a library is complete, and as the consequences of retransformation of the library on these probabilities are presented. (C) 2005 Elsevier B.V. All rights reserved.
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