期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 49, 期 6, 页码 551-559出版社
WILEY
DOI: 10.1002/mnfr.200500030
关键词
amaranth; ferulic acid; feruloylated oligosaccharides; nuclear magnetic resonance; pectins
The association of ferulic acid, an alkali-extractable phenolic acid in amaranth (Amaranthus caudatus L., Amaranthaceae) insoluble fiber (trans-ferulic acid: 620 mu g (center dot) g(-1), cis-ferulic acid: 203 pg center dot g(-1)), and non-starch polysaccharides was investigated. Enzymatic hydrolysis of insoluble amaranth fiber released several feruloylated oligosaccharides that were separated using Sephadex LH-20-chromatography and reversed phase-high performance liquid chromatography (RP-HPLC). Three compounds were unambiguously identified: O-(6-O-trans-feruloyl-beta-D-galactopyranosyl)-(1 -> 4)-D-galactopyranose, O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1 -> 5)-L-arabinofuranose, and O-alpha-L-arabinofuranosyl-(1 -> 3) 3)-O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1 -> 5)-L-arabinofuranose. These feruloylated oligosaccharides show that ferulic acid is predominantly bound to pectic arabinans and galactans in amaranth insoluble fiber. 5-O-trans-Feruloyl-L-arabinofuranose was the only compound isolated in pure form from an acid hydrolyzate. This compound may have its origin from pectic arabinans but also from arabinoxylans.
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