期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 41, 期 -, 页码 225-231出版社
WILEY
DOI: 10.1042/BA20040144
关键词
Arthrobacter ureafaciens sialidase (neuraminidase) gene; asialoproteins; desialylation; erythropoietin; Ser-Xaa-Asp-Xaa-Gly-Xaa-Thr-Trp (Asp box); twin-arginine translational signal (Tat signal)
Sialidases have recently been used in the processing of clinically relevant asialoproteins. The Arthrobacter ureafaciens sialidase (EC 3.2. 1. 18) exhibits broad substrate specificity and is often used in such applications. We have employed an expression cloning strategy to isolate the A. ureafaciens sialiclase. The clone encodes a 990-amino-acid 104 kDa open-reading-frame protein containing three domains: an N-terminal catalytic domain, a linker domain with an immunoglobulin-like fold and a C-terminal domain of unknown function. Expression in Escherichia coli indicates that the sialiclase promoter was active in E. coli. Overexpression in E. coli resulted in several truncated forms. A 54 kDa truncated variant was generated, expressed and purified, and its feasibility for use in an erythropoietin desialylation process was demonstrated.
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