期刊
BIOMEDICAL CHROMATOGRAPHY
卷 19, 期 5, 页码 375-378出版社
WILEY
DOI: 10.1002/bmc.460
关键词
puerarin; Pueraria lobata isoflavone; yufengningxin tablets; self-microemulsifying drug delivery systems; bioavailability; HPLC-MS
To evaluate the bioavailability of puerarin from Pueraria lobata isoflavone self-microemulsifying drug delivery systems (SMEDDS) and Yufengningxin tablets, a rapid and specific liquid chromatography-mass spectrometric method was developed and validated to determine puerarin in rabbit serum. The analyte was extracted from serum samples by precipitating the serum proteins, separated on a Dianionsil C-18 column and detected by mass spectrometry with an electrospray ionization interface. 4-Hydroxybenzaidehyde was used as the internal standard. The method has a limit of quantitation of 10 ng/mL using 200 mu L serum. The intra-day relative standard deviations (RSDs) ranged from 3.7 to 6.9% and inter-day RSDs were within 6.5%. After administration of SMEDDS and tablets to rabbits, a significant difference was observed in main pharmacokinetic parameters of t(max), C-max and AUC(0-infinity) between SMEDDS and tablets, and a 2.2-fold increase in the relative bioavailability of puerarin was observed with the SMEDDS compared with Yufengningxin tablets. It was concluded that the absorption of puerarin from Piteraria lobata isoflavone SMEDDS was enhanced. Copyright (c) 2004 John Wiley & Sons, Ltd.
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