期刊
JOURNAL OF BACTERIOLOGY
卷 187, 期 12, 页码 4005-4014出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.187.12.4005-4014.2005
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资金
- NIAID NIH HHS [AI59698, R01 AI042347, AI42347, R21 AI059698, R37 AI042347] Funding Source: Medline
- NIDDK NIH HHS [P30 DK034928, P30DK-34928] Funding Source: Medline
Numerous small untranslated RNAs (sRNAs) have been identified in Escherichia coli in recent years, and their roles are gradually being defined. However, few of these sRNAs appear to be conserved in Vibrio cholerae, and both identification and characterization of sRNAs in V. cholerae remain at a preliminary stage. We have characterized one of the few sRNAs conserved between E. coli and V cholerae: RyhB. Sequence conservation is limited to the central region of the gene, and RyhB in V. cholerae is significantly larger than in E. coli. As in E. coli, V. cholerae RyhB is regulated by the iron-dependent repressor Fur, and it interacts with the RNA-binding protein Hfq. The regulons controlled by RyhB in V. cholerae and E. coli appear to differ, although some overlap is evident. Analysis of gene expression in V. cholerae in the absence of RyhB suggests that the role of this sRNA is not limited to control of iron utilization. Quantitation of RyhB expression in the suckling mouse intestine suggests that iron availability is not limiting in this environment, and RyhB is not required for colonization of this mammalian host by V. cholerae.
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