期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 61, 期 8, 页码 1821-1833出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2004.06.054
关键词
xanthene dyes; aggregation; protic solvents; surfactants
类别
The effect of solvents, halo substituents and surfactants on the aggregation of xanthene dyes (fluorescein and eosin Y) has been investigated. It has been found that polar protic solvents promote the aggregation of both the dyes while polar aprotic solvents hinder the aggregation process. Apolar solvents can disintegrate the aggregates previously formed in aqueous medium. The nature of the driving forces for aggregation in the two dyes is different. Surfactants bearing charge opposite to the dye molecules facilitate aggregation. These observations bear direct relevance to the possible use of fluorescein and eosin Y as quantum yield standards, photosensitizers, laser dyes and biological labels. (c) 2004 Elsevier B.V. All rights reserved.
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