期刊
NATURE REVIEWS MICROBIOLOGY
卷 3, 期 6, 页码 499-504出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nrmicro1162
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资金
- Natural Environment Research Council [NER/A/S/2002/00876, NE/B505362/1] Funding Source: researchfish
Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as C-13, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and C-13-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics.
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