期刊
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
卷 66, 期 6, 页码 213-221出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pmpp.2005.06.005
关键词
Botrytis cinerea; cellulase; endo-beta-1,4-glucanase
The cel5A gene, coding for an extracellular endo-beta-1,4-glucanase, has been cloned from the phytopathogenic fungus Botrytis cinerea. The gene contains only one 62-bp intron and encodes a putative protein of 424 amino acids with a signal peptide, a family-5 glycosyl hydrolase domain, and a serine-threonine-rich domain of unknown function. The levels of cel5A transcript and extracellular beta-1,4-glucanase activity were regulated by the carbon source in the same way: both were induced by carboxymethylcellulose and repressed by glucose. The cel5A mRNA could also be detected during the infection of tomato leaves by B. cinerea. Disruption of cel5A results in a strain with identical virulence as the wild-type on tomato leaves or gerbera petals, with no significant reduction in the extracellular beta-1,4-glucanase activity, and with similar growth rate with carboxymethylcellulose or Avicel as the only carbon sources. (c) 2005 Elsevier Ltd. All rights reserved.
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