Expression of P2X4 receptor by lesional activated microglia during formalin-induced inflammatory pain

P2X(4) receptor (P2X(4)R) is an ion channel gated by adenosine 5'-triphosphate. Here we report the presence and the distribution of P2X(4)R in rat spinal cord by immunohistochemical analysis in an inflammatory pain model. Peripheral inflammation was induced by subcutaneous injection of 4% formalin into the rat hindpaw. Morphology, spatial localization, and activation state of P2X(4)R(+) cells were described at 1, 5, 7, 14, and 28 days after injury. In normal and saline treated control rats, P2X(4)R was rarely seen. After formalin administration, an increase of P2X(4)R(+) microglia were observed in the spinal cord dorsal horn on the side ipsilateral to the injection, reaching maximal levels by day 7, and then decreasing to normal levels by day 14. This implicates a role of P2X(4)R in the spinal inflammatory pain process. Furthermore, formalin-induced region-specific increase in activated microglia was confirmed by ED1 and endothelial monocytes activating polypeptide II (EMAP-II) expression. In conclusion, this is the first demonstration that P2X(4)R is expressed by microglia in the inflammatory pain. (C) 2005 Elsevier B.V. All rights reserved.