期刊
NEUROSCIENCE RESEARCH
卷 52, 期 2, 页码 132-138出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neures.2005.02.004
关键词
slice preparation; cortex; fluo-4; Oregon green; spike; microcircuitry; action potential; circuit; spinning disk; confocal microscope
Bulk loading of calcium indicators has provided a unique opportunity to reconstruct the activity of cortical networks with single-cell resolution. Here we describe the detailed methods of bulk loading of AM dyes we developed and have been improving for imaging with a spinning disk confocal microscope. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
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