Tumor suppressor VDUP1 increases p27kip1 stability by inhibiting JAB1

Vitamin D-3 up-regulated protein 1 (VDUP1) is a stress-response gene that is up-regulated by 1,25(OH)(2)D-3 in many cells. It has been reported that VDUP1 expression is reduced in many tumor cells and the enforced expression of VDUP1 inhibits cell proliferation by arresting cell cycle progression. Here, we found that VDUPI-/- fibroblast cells proliferated more rapidly compared with wild-type cells with reduced expression of p27(kip1), a cyclin-dependent kinase inhibitor. JAB1 is known to interact with p27(kip1) and to decrease the stability of p27(kip1). VDUP1 interacted with JAB1 and restored JAB1-induced suppression of p27 kipl stability. In this process, VDUP1 blocked the JAB1-mediated translocation of p27 kipl from the nucleus to the cytoplasm. In addition, VDUP1 inhibited JAB1-mediated activator protein-1 activation and cell proliferation. Taken together, these results indicate that VDUP1 is a novel factor of p27(kip1) stability via regulating JAB1.