期刊
NATURE MATERIALS
卷 4, 期 6, 页码 460-464出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmat1392
关键词
-
类别
资金
- NIDCR NIH HHS [R30 DE 13033] Funding Source: Medline
Non-viral gene vectors are commonly used for gene therapy(1-3) owing to safety concerns with viral vectors(4). However, nonviral vectors are plagued by low levels of gene transfection and cellular expression(1,2). Current efforts to improve the efficiency of non-viral gene delivery are focused on manipulations of the delivery vector(5-12), whereas the influence of the cellular environment in DNA uptake is oft en ignored. The mechanical properties ( for example, rigidity) of the substrate to which a cell adheres have been found to mediate many aspects of cell function including proliferation, migration and differentiation(13-17), and this suggests that the mechanics of the adhesion substrate may regulate a cell's ability to uptake exogeneous signalling molecules. In this report, we present a critical role for the rigidity of the cell adhesion substrate on the level of gene transfer and expression. The mechanism relates to material control over cell proliferation, and was investigated using a fluorescent resonance energy transfer (FRET) technique(18-21). This study provides a new material-based control point for non-viral gene therapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据